Typhoid IgG/IgM Antibody Test Kit (Immunochromatographic Assay) adopts the colloidal gold method to qualitatively detect the antibody of typhoid bacillus (lipopolysaccharide antigen and outer membrane protein antigen) in human serum / plasma, which is suitable for the early auxiliary diagnosis of typhoid infection.
Typhoid IgG/IgM Antibody Test Kit (Immunochromatographic Assay) is a lateral flow chromatography immunoassay. The test cassette consists of: 1) a burgundy colored conjugate pad containing recombinant S. typhoid H antigen and O antigen conjugated with colloid gold (Typhoid conjugates) and rabbit IgG ‐gold conjugates, 2) a nitrocellulose membrane strip containing two test bands (IgG and IgM bands) and a control band (C band). The IgM band is pre-coated with monoclonal anti-human IgM for the detection of IgM anti-S. typhi, IgG band is pre-coated with reagents for the detection of IgG anti-S.typhi , and the C band is pre-coated with goat anti rabbit IgG.
|Materials / provided||Quantity(1 Test/Kit)
|Test Kit||1 test||5 tests||25 tests|
|Buffer||1 bottle||5 bottles||25/2 bottles|
|Dropper||1 piece||5 piece||25 piece|
|Specimen Transport Bag||1 piece||5 pcs||25 pcs|
|Disposable Lancet||1 piece||5 pcs||25 pcs|
|Instructions For Use||1 piece||1 piece||1 piece|
|Certificate of Conformity||1 piece||1 piece||1 piece|
Collect human Serum/Plasma/Whole blood properly.
1. Remove an extraction tube from the kit and a test box from the film bag by tearing the notch. Open the inspection card aluminum foil bag. Remove the test card and place them horizontally on a table.
2. Use a disposable pipette, transfer 4μL serum (or plasma), or 4μL whole blood into the sample well on the test cassette.
3.Open the buffer tube by twisting off the top. Put 3 drops (about 80 μL) of assay diluent into the assay diluent well round shaped. Start counting.
Read the result at 15 min. Results after 20 minutes are invalid.
Only the quality control line C appears and the detection lines G and M do not show, it means that no Typhoid antibody is detected and the result is negative.
If both the quality control line C and the detection line M appear= the Typhoid IgM antibody is detected, and the result is positive for the IgM antibody.
If both the quality control line C and the detection line G appear= the Typhoid IgG antibody is detected and the result is positive for the IgG antibody.
If both the quality control line C and the detection lines G and M appear= the Typhoid IgG and IgM antibodies are detected, and the result is positive for both IgG and IgM antibodies.
If the quality control line C cannot be observed, results will be invalid
regardless of whether a test line shows, and the test should be repeated.
|Product Name||Cat. No||Size||Specimen||Shelf Life||Trans. & Sto. Temp.|
|Typhoid IgG/IgM Antibody test kit (Immunochromatographic Assay)||B023C-01||1test/kit||Serum/Plasma/Whole Blood||18 Months||2-30℃ / 36-86℉|